Optimizing a Method for Separating Chiral Compounds by Capillary Electrophoresis

Abstract

This work describes the development of a capillary electrophoresis method for separation of chiral compounds using several (cyclodextrin derivatives (-CDs) in the mobile phase. The aims of this work are to demonstrate the effectiveness of -CDs to separate the chiral compounds and to show how the separations are affected by various buffers and different pH values. The results show that the chiral drugs atropine (alkaloid antidote for cholinesterase-inhibiting compounds), trolox, methylphenyloxazolidinone, ephedrine, and pseudoephedrine can be separated with excellent resolutions by using sulfobutylether (-CDs. Dansyl-DL-amino acids can be separated within 5 min in aqueous solutions. The greatest resolution between L and D amino acids is found at pH 3.0.