Quantification of the Cellular Uptake of Cell‐Penetrating Peptides by MALDI‐TOF Mass Spectrometry

Abstract

The ins and outs of peptides: The internalization efficiencies of three cell-penetrating peptides (CPPs; penetratin, (Arg)9, and Tat48–59) have been measured by MALDI-TOF MS (see scheme). The method leads to direct detection of the CPPs and allows unambiguous discrimination between membrane-bound and internalized CPP. Furthermore, the cellular uptake efficiencies of several CPPs can be compared in a single experiment. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2005/z500477_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.