Arthrobacter Endo‐β‐<i>N</i>‐Acetylglucosaminidase Shows Transglycosylation Activity on Complex‐Type <i>N</i>‐Glycan Oxazolines: One‐Pot Conversion of Ribonuclease B to Sialylated Ribonuclease C

Abstract

Transforming protein glycosylation: The Arthrobacter endo-β-N-acetylglucosaminidase (Endo-A) was found to transglycosylate complex N-glycan oxazolines without product hydrolysis. Given its high activity in hydrolyzing high-mannose-type N-glycans, the newly discovered property enables a one-pot switch of glycans in a glycoprotein, as shown by the conversion of heterogeneous RNase B to homogeneous sialylated RNase C.