Skeletal Cell Fate Decisions Within Periosteum and Bone Marrow During Bone Regeneration

TLDR

Bone repair requires mobilization of adult skeletal stem cells that are believed to originate from both bone marrow and periosteum. The study aimed to delineate the cellular contributions of bone marrow and periosteum to bone healing in vivo and to evaluate how the tissue environment influences their differentiation. Lineage tracing was employed to track the fate of cells derived from periosteum, bone marrow, and the endosteum subcompartment. Periosteal injuries heal by endochondral ossification and bone marrow injuries by intramembranous ossification, with progenitors recruited locally from both tissues; periosteum predominantly generates chondrocytes while both tissues produce osteoblasts, and intrinsic and environmental signals modulate these distinct fate decisions during.

Abstract

Bone repair requires the mobilization of adult skeletal stem cells/progenitors to allow deposition of cartilage and bone at the injury site. These stem cells/progenitors are believed to come from multiple sources including the bone marrow and the periosteum. The goal of this study was to establish the cellular contributions of bone marrow and periosteum to bone healing in vivo and to assess the effect of the tissue environment on cell differentiation within bone marrow and periosteum. Results show that periosteal injuries heal by endochondral ossification, whereas bone marrow injuries heal by intramembranous ossification, indicating that distinct cellular responses occur within these tissues during repair. [corrected] Next, lineage analyses were used to track the fate of cells derived from periosteum, bone marrow, and endosteum, a subcompartment of the bone marrow. Skeletal progenitor cells were found to be recruited locally and concurrently from periosteum and/or bone marrow/endosteum during bone repair. Periosteum and bone marrow/endosteum both gave rise to osteoblasts, whereas the periosteum was the major source of chondrocytes. Finally, results show that intrinsic and environmental signals modulate cell fate decisions within these tissues. In conclusion, this study sheds light into the origins of skeletal stem cells/progenitors during bone regeneration and indicates that periosteum, endosteum, and bone marrow contain pools of stem cells/progenitors with distinct osteogenic and chondrogenic potentials that vary with the tissue environment.