International Journal of Systematic Bacteriology · 1997 · 91 citations · 13 references
Life SciencesMicrobiologyMolecular BiologyComparative MicrobiologyMicrobial GeneticsMicrobial Taxonomy16S Ribosomal Ribonucleic Acid AnalysisBacterial ClassificationDna SequencingPhylogenetic ClassificationProkaryotic ClassificationTaxonomic Classification16S Ribosomal Ribonucleic Acid (Rrna) Sequencing16S Ribosomal Ribonucleic Acid Gene Analysis16S Ribosomal Ribonucleic Acid Gene Sequencing16S Ribosomal Ribonucleic Acid Sequence Analysis16S Ribosomal Rna Classification16S Ribosomal Rna Gene Analysis16S Rrna Gene PhylogenyMolecular Identification Of Strains
The primary structures of the 16S rRNA genes of the type strains of Lactobacillus casei and related taxa were determined by PCR DNA-sequencing methods. The sequences of Lactobacillus casei, Lactobacillus zeae, Lactobacillus paracasei, and Lactobacillus rhamnosus were different. The Knuc values ranged from 0.0040 to 0.0126. On the basis of the Knuc values and the levels of DNA-DNA relatedness among the strains of these species, the L. casei-related taxa should be classified in the following three species: L. zeae, which includes the type strains of L. zeae and L. casei; a species that includes the strains of L. paracasei and L. casei ATCC 334; and L. rhamnosus.
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