Cross‐amplification tests of ungulate primers in roe deer (<i>Capreolus capreolus</i>) to develop a multiplex panel of 12 microsatellite loci

Abstract

Abstract Cross‐amplification permits transposition of microsatellite markers to closely related species. Multiplexing reduces time and cost further, exploiting simultaneous amplification of several primers. In cross‐amplification tests of 55 ungulate primers in roe deer, 39 gave specific amplification products and 20 were polymorphic. Twelve primers were retained to form a multiplex kit. In 30 roe deer, average allelic diversity was 5.67 (range: 2–15), expected heterozygosity was 0.664 and mean polymorphic information content (PIC) value was 0.605. Probability of identity was P ID = 5 × 10 −11 and probability of exclusion for parentage studies was P E1 = 0.98856 and P E2 = 0.99952.