Antidiarrheal Activity of the Aqueous Extract of<i>Punica granatum</i>. (Pomegranate) Peels

Abstract

The antidiarrheal effects of the aqueous extract of Punica granatum. L. (Punicaceae) peels were evaluated in rats. Studies were carried out on the isolated rat ileum, gastrointestinal motility in vivo., and on castor oil–induced diarrhea in rats. The results revealed that the extract exhibited a concentration-dependent inhibition of the spontaneous movement of the isolated rat ileum and attenuated acetylcholine-induced contractions. The extract (100, 200, 300, and 400 mg/kg) also caused a dose-dependent decrease of gastrointestinal transit and markedly protected rats against castor oil–induced diarrhea enteropooling. The intraperitoneal injection LD50 of the extract was found to be 1321 ± 15 mg/kg in mice. A preliminary phytochemical screening of the aqueous extract of Punica granatum. peels gave positive tests for tannins, flavonoids, and alkaloids. The results obtained showed that the aqueous extract of Punica granatum. peels may contain some biologically active principles that may be active against diarrhea, and this may be the basis for its traditional use for gastrointestinal disorders.