Differentiation of Genes Coding for <i>Escherichia coli</i> Verotoxin 2 and the Verotoxin Associated with Porcine Edema Disease (VTe) by the Polymerase Chain Reaction

Abstract

Two sets of synthetic oligonucleotide primers were used in a polymerase chain reaction adaptation to distinguish the closely related genes for type 2 verotoxin (VT2 or Shiga-like toxin [SLT-II]) and the verotoxin associated with porcine edema disease (VTe or SLT-II variant [SLT-IIv]) in Escherichia coli.