Adaptation of the Micronucleus Technique in Allium Cepa, For Mutagenicity Analysis of the Jamari River Valley, Western Amazon, Brazil

Abstract

This study aimed to make technique adjustment of micronuclei in Allium cepa, recommended by Fiskejo [1], by replacing methanol and Giemsa by triarilmetano the 0,1%, xantenos the 0,1% and tiazinas the 0,1%. We obtained good results with the replacement, as its efficiency in stain nucleic acids, obtaining a good visualization of cells, mitotic index, micronuclei, anaphase and telophase bridges, and this methodology is indicated in future examinations of mutagenicity in the river Vale do Jamari.