One-Hybrid Screens at the <i>Saccharomyces cerevisiae HMR</i> Locus Identify Novel Transcriptional Silencing Factors

Abstract

In Saccharomyces cerevisiae, genes located at the telomeres and the HM loci are subject to transcriptional silencing. Here, we report results of screening a Gal4 DNA-binding domain hybrid library for proteins that cause silencing when targeted to a silencer-defective HMR locus.