Journal of Bacteriology · 1987 · 2.3K citations · 17 references
Life SciencesMicrobiologyMolecular BiologyEnzymologyMicrobial GeneticsProtein BiochemistryEnzyme CharacterizationProtein Sequence AnalysisAmino AcidsEscherichia ColiMolecular Weight DeterminationOsmotic ShockSignal Peptide AnalysisSignal Peptide MotifSignal Peptide Prediction
The iap gene in Escherichia coli is responsible for the isozyme conversion of alkaline phosphatase. We analyzed the 1,664‑nucleotide sequence of a chromosomal DNA segment that contained the iap gene and its flanking regions. The iap gene encodes a 345‑amino‑acid protein (~37.9 kDa) with an N‑terminal signal peptide, yielding two membrane‑associated forms (full‑length and processed) that lack isozyme‑converting activity in osmotic‑shock fluid of multicopy plasmid‑bearing cells.
The iap gene in Escherichia coli is responsible for the isozyme conversion of alkaline phosphatase. We analyzed the 1,664-nucleotide sequence of a chromosomal DNA segment that contained the iap gene and its flanking regions. The predicted iap product contained 345 amino acids with an estimated molecular weight of 37,919. The 24-amino-acid sequence at the amino terminus showed features characteristic of a signal peptide. Two proteins of different sizes were identified by the maxicell method, one corresponding to the Iap protein and the other corresponding to the processed product without the signal peptide. Neither the isozyme-converting activity nor labeled Iap proteins were detected in the osmotic-shock fluid of cells carrying a multicopy iap plasmid. The Iap protein seems to be associated with the membrane.
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A system for shotgun DNA sequencing
Joachim Messing, Roberto Crea, Peter H. Seeburg · Nucleic Acids Research · 1981 · 2.6K citations · Full text