Lysobacter korlensis sp. nov. and Lysobacter bugurensis sp. nov., isolated from soil

Abstract

Two Gram-reaction-negative, rod-shaped, gliding, yellow-pigmented bacterial strains, designated ZLD-17(T) and ZLD-29(T), were isolated from arid soil samples collected from Xinjiang Province, north-west China, and subjected to analysis using a polyphasic taxonomic approach. Both novel strains required 1.0-2.0 % (w/v) sea salts for optimal growth. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these two strains belong to the genus Lysobacter within the class Gammaproteobacteria. Strain ZLD-17(T) showed highest 16S rRNA gene sequence similarities to Lysobacter capsici KCTC 22007(T) (96.9 %), Lysobacter spongiicola DSM 21749(T) (96.8 %) and Lysobacter koreensis KCTC 12204(T) (96.8 %), whereas strain ZLD-29(T) showed highest sequence similarities to Lysobacter niastensis DSM 18481(T) (96.0 %) and Lysobacter enzymogenes DSM 2043(T) (95.9 %). 16S rRNA gene sequence similarity between ZLD-17(T) and ZLD-29(T) was 96.1 %. The DNA G+C contents of strains ZLD-17(T) and ZLD-29(T) were 67.9 and 68.2 mol%, respectively. The major cellular fatty acids of both strains were summed feature 3 (iso-C₁₅:₀ 2-OH and/or C₁₆:₁ω7c), iso-C₁₇:₁ω9c, iso-C₁₆:₀, C₁₆:₀ and iso-C₁₁:₀ 3-OH; their predominant isoprenoid quinone was Q-8 and their major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Based on their phenotypic characteristics, phylogenetic position as determined by 16S rRNA gene sequence analysis and chemotaxonomic data, strains ZLD-17(T) ( = CCTCC AB 207174(T) = KCTC 23076(T)) and ZLD-29(T) ( = CCTCC AB 207175(T) = KCTC 23077(T)) represent two novel species of the genus Lysobacter, for which the names Lysobacter korlensis sp. nov. and Lysobacter bugurensis sp. nov. are proposed, respectively.