The essentiality of histo- and cytochemical studies of skeletal muscle in the investigation of neuromuscular disease

TLDR

Cytochemical changes in skeletal muscle may not always correlate with fine‑structural alterations, and combining cytochemistry with electron microscopy is limited by the scarcity of suitable reactions and practical constraints. The study aims to demonstrate that histo‑ and cytochemical techniques with light microscopy are essential for diagnosing and researching neuromuscular disease. Histo‑ and cytochemical methods detect both generalized loss of enzyme activity and localized fiber‑specific changes, and when refined can reveal functional activity of fine‑structural muscle components. Six recent studies illustrate cytopathologic changes detectable by these techniques, enabling predictions of fine‑structural alterations that electron microscopy would confirm. © 1962, Neurology 12:778‑794, American Academy of Neurology.

Abstract

American Academy of Neurology Dedicated to advancing the art and science of neurology, and thereby promoting the best possible care for patients with neurological disorders A Celebration of the First 50 years of the American Academy of Neurology Neurology 1962;12:778-794 In the investigation of neuromuscular disease, histologic examination of skeletal muscle biopsies is generally accepted as an important part both of routine diagnostic procedures and of more specialized research. The purpose of the present report is to establish that the use of histo- and cytochemical technics combined with light microscopy is also an essential aspect of both types of investigation. Substantiating evidence is presented in the form of 6 examples, chosen from recent studies, of cytopathologic changes in neuromuscular disease as demonstrated by these technics. Two fundamental types of abnormalities in skeletal muscle are detectable with histo- and cytochemical technics: (a) a generalized change of a given reaction, for example, loss of phosphorylase activity, and (b) a localized change of activity confined either to a specific type of muscle fiber (as in the new myopathy with rod-shaped structures) or to certain regions of individual muscle fibers (as in target fibers and central core disease). Further, if the cytochemical technics are sufficiently refined, they may be used to demonstrate the functional activity of fine structural components of the muscle cell. On the basis of such data, predictions can often be made as to the fine structural changes which electron-microscopy will reveal. It must be understood, though, that some cytochemical changes might not be accompanied by changes of fine structure and vice versa. Cytochemistry combined with electron-microscopy would give even more detailed information, but this approach has 2 major limitations: (a) only a very few cytochemical reactions are as yet refined enough to be used with electron-microscopy and(b) study of a large …