Nucleic Acids Research · 1998 · 18 citations · 31 references
Life SciencesBiochemistryMolecular BiologyNucleic Acid BiochemistryNucleic Acid ChemistryStructural BiochemistryDna ChemistryDna StructureNucleic Acid StructureOligonucleotide ChemistryRibonucleic Acid (Rna) ChemistryRna StructureDna Double HelixDouble-stranded Dna StructureDuplex StructuresNucleic Acid Base PairingOligonucleotide HybridizationRibonucleic Acid (Rna) StructureRibonucleotide ChemistryRna Hybridization
Arabinonucleic acid, the 2'-stereoisomer of RNA, was tested for its ability to recognize double-helical DNA, double-helical RNA and RNA-DNA hybrids. A pyrimidine oligoarabinonucleotide (ANA) was shown to form triple-helical complexes only with duplex DNA and hybrid DNA (Pu):RNA (Py) with an affinity that was slightly lower relative to the corresponding pyrimidine oligodeoxynucleotide (DNA) third strand. Neither the ANA nor DNA third strands were able to bind to duplex RNA or hybrid RNA (Pu):DNA (Py). In contrast, an RNA third strand recognized all four possible duplexes (DD, DR, RD and RR), as previously demonstrated. Such an understanding can be applied to the design of sequence-selective oligonucleotides which interact with double-stranded nucleic acids and emphasizes the role of the 2'-OH group as a general recognition and binding determinant of RNA.
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Trung Le Doan, Danièle Praseuth, Nourcddine Habhoub et al. · Nucleic Acids Research · 1987 · 490 citations · Full text