The Journal of Infectious Diseases · 2014 · 51 citations · 39 references
Life SciencesBiochemistryInfectious DiseasesCellular MetabolismInnate ImmunityMetabolismLipid BiosynthesisLipid MetabolismParasitic Disease PathogenesisParasitic PathogenesisProtozoan Host-parasite InteractionsProtozoan PathogenesisArachidonic Acid BiosynthesisHost Immune Response ModulationHost Immune Response To ProtozoaHost-protozoan Metabolic InteractionsLeishmaniasis PathogenesisMacrophage Responses To LeishmaniaPathogen-host InteractionsProstaglandin Production
Lipid bodies (LB; lipid droplets) are cytoplasmic organelles involved in lipid metabolism. Mammalian LBs display an important role in host-pathogen interactions, but the role of parasite LBs in biosynthesis of prostaglandin F2α (PGF2α) has not been investigated. We report herein that LBs increased in abundance during development of Leishmania infantum chagasi to a virulent metacyclic stage, as did the expression of PGF2α synthase (PGFS). The amount of parasite LBs and PGF2α were modulated by exogenous arachidonic acid. During macrophage infection, LBs were restricted to parasites inside the parasitophorous vacuoles (PV). We detected PGF2α receptor (FP) on the Leishmania PV surface. The blockage of FP with AL8810, a selective antagonist, hampered Leishmania infection, whereas the irreversible inhibition of cyclooxygenase with aspirin increased the parasite burden. These data demonstrate novel functions for parasite-derived LBs and PGF2α in the cellular metabolism of Leishmania and its evasion of the host immune response.
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Lipid Droplets Finally Get a Little R-E-S-P-E-C-T
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