Concepedia

Characterization of beta-lactamases in situ on polyacrylamide gels

Christine C. Sanders, William E. Sanders, Ellen Smith Moland

Antimicrobial Agents and Chemotherapy · 1986 · 81 citations · 2 references

DOI

Concepts

Abstract

An inhibitor-based characterization system which allowed the identification of beta-lactamases after isoelectric focusing on polyacrylamide gels was developed. This system, using potassium clavulanate and oxacillin, distinguished type I chromosomally mediated enzymes from other beta-lactamases of gram-negative bacteria.

References

2

The Use of Analytical Isoelectric Focusing for Detection and Identification of  -Lactamases

M. Matthew, Anne Harris, Matthew J. Marshall et al. · Journal of General Microbiology · 1975 · 691 citations

, , +13

Comparison of polyacrylamide and agarose gel thin-layer isoelectric focusing for the characterization of beta-lactamases

Cecilia Vecoli, F E Prevost, J J Ververis et al. · Antimicrobial Agents and Chemotherapy · 1983 · 54 citations

, , +12