Simulation of the Electron Transfer between the Tetraheme Subunit and the Special Pair of the Photosynthetic Reaction Center Using a Microstate Description

Abstract

Charge transfer through biological macromolecules is essential for many biological processes such as, for instance, photosynthesis and respiration. Protons or electrons are transferred between titratable residues or redox-active cofactors, respectively. Transfer rates between these sites depend on the current charge configuration of neighboring sites. Here, we formulate the kinetics of charge-transfer systems in a microstate formalism. A unique transfer rate constant can be assigned to the interconversion of microstates. Mutual interactions between sites participating in the transfer reactions are naturally taken into account. The formalism is applied to the kinetics of electron transfer in the tetraheme subunit and the special pair of the reaction center of Blastochloris viridis. It is shown that continuum electrostatic calculations can be used in combination with an existing empirical rate law to obtain electron-transfer rate constants. The re-reduction kinetics of the photo-oxidized special pair simulated in a microstate formalism is shown to be in good agreement with experimental data. A flux analysis is used to follow the individual electron-transfer steps.