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Complete Nucleotide Sequence of the Escherichia coli Plasmid pBR322
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1979
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GeneticsBacteriologyMolecular BiologyGenomicsSequence AlignmentDna-sequencing TechniqueCloningDna SequencingSequence AnalysisDna ReplicationMolecular MicrobiologyDna StructureBioinformaticsNatural SciencesComplete Nucleotide SequenceVector Pbr322MicrobiologyMedicineSequence AssemblyMicrobial Genetics
I have determined the 4362-nucleotide-pair sequence of the plasmid cloning vector pBR322 using the DNA-sequencing technique of Maxam and Gilbert (1977). The DNA structure has several interesting features that lead to testable predictions.