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Isolation and culture of endothelial cells from the lungs of small animals.
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1979
Year
Small AnimalsEndothelial CellsLung InflammationImmunologyCellular PhysiologyAngiogenesisPulmonary PharmacologyEndothelial Cell PathobiologyAnimal PhysiologyPulmonary CirculationVascular BiologyNeovascularizationSurface ReplicasCell BiologyPhysiologyEndothelial DysfunctionVeterinary SciencePulmonary PhysiologyTissue CultureMedicineExtracellular Matrix
Techniques are descirbed for the isolation and culture of endothelial cells from the lungs of small animals. The cells are collected by retrograde perfusion of blood-free lungs with buffered saline containing collagenase. The cells are characterized by light microscopy, electron microscopy of thin sections and surface replicas, and by the presence of angiotensin-converting enzyme (ACE). ACE was assayed using 3H-benzoyl-Phe-Ala-Pro as substrate and was localized by indirect immunofluorescence using guinea pig endothelial cells incubated with rabbit antibodies to guinea pig lung ACE followed by goat anti-rabbit globulins conjugated to fluorescein. Thus, endothelial cultures can be established using small animals commonly employed in studies of pulmonary processing of vasoactive substances.