A New Flow‐Injection Chemiluminescence Method for the Determination of Acyclovir and Gancyclovir

Abstract

Abstract A rapid and sensitive flow‐injection chemiluminescence (FI‐CL) method, which is based on the CL intensity that generated from the redox reaction of Ce(IV)‐rhodamine B in H2SO4 medium, for the determination of acyclovir and gancyclovir is described. For acyclovir, the determination range is 3×10−8 g mL−1–7×10−5 g mL−1, with 1.56×10−8 g mL−1 as its determination limit. During 11 repeated measurements for 1×10−6 g mL−1 acyclovir, the relative standard deviation was 2.08%. For gancyclovir, the determination range was 5×10−8 g mL−1–7×10−5 g mL−1, with 2.35×10−8 g mL−1 as its determination limit. The relative standard deviation is 2.83% with 11 repeated measurements of 1×10−6 g mL−1 gancyclovir. This method can be successfully used to determine the content of acyclovir and gancyclovir in injections, acyclovir in eye drops, and, maybe, also for other ciclovirs.