Mutant DNA Polymerase for Improved Detection of Single‐Nucleotide Variations in Microarrayed Primer Extension

Abstract

Mutant detects mutation. A mutant DNA polymerase, which was derived from Pyrococcus furiosus DNA polymerase (M2 in figure), was found to have improved single-nucleotide discrimination properties, and was used for selective microarrayed-primer extensions. This approach allowed multiplexed SNP detection.