Improved in vitro culture and micropropagation of different Melissa officinalis L. genotypes

Abstract

Melissa officinalis L. is widely cultivated in the world and Iran as well, due to its useful application in medicine. In order to establish a stable and high frequency of regeneration system with 4 landrace collected from different climate in Iran, major parameters such as regeneration rate, rooting percentage, shooting and proliferation rate were investigated. Statistical analysis of results showed that BAP in combination with NAA had the highest regeneration in shoot tips explants. NAA in combination with IAA and Kinetin had the best response to callus induction. Also 1 mgl/l NAA had the higher response to rooting than other’s used auxins. Explants derived from Hamedan landrace had the highest potential for regeneration. This is the first report on high frequency in vitro regeneration in four diverse landraces of M. officinalis selected from various climates regions in Iran.