Detection of malignant <scp>B</scp> lymphocytes by <scp>PCR</scp> clonality assay using direct lysis of cerebrospinal fluid and low volume specimens

Abstract

PCR clonality with direct cell lysis of CSF is feasible, and it may overcome the limitation of DNA isolation. This PCR method may be particularly useful for small volume and low cell CSF when flow cytometry is inconclusive.