Continuous Fluorometric Assays for Acetylcholinesterase Activity and Inhibition with Conjugated Polyelectrolytes

Abstract

Quick but sensitive: A super-quenched fluorogenic complex of a cationic acetylcholine (ACh) derivative with an energy-acceptor tag and an anionic water-soluble conjugated polymer forms the basis of a highly effective fluorescence turn-on assay for studying the enzyme kinetics and inhibition of acetylcholinesterase (AChE; see schematic representation of the assay). FRET=fluorescence resonant energy transfer. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2007/z701724_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.