SIMULTANEOUS DETERMINATION OF PARACETAMOL, CAFFEINE, DOMPERIDONE, ERGOTAMINE TARTRATE, PROPYPHENAZONE, AND DROTAVERINE HCL BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Abstract

A high performance liquid chromatographic (HPLC) method was developed for the simultaneous determination of paracetamol, (acetaminophen), caffeine, domperidone, ergotamine tartrate, propyphenazone, and drotaverine HCl. The chromatography was performed in the isocratic mode on a Hypersil C18 BDS column using a mobile phase consisting of 0.02 mole L−1 tetrabutylammonium bisulphate and methanol (100:45, v/v) at 50°C using UV detection at 210 nm. The method was validated by specificity, linearity, limit of detection, and limit of quantification, accuracy, and precision for the aforementioned drugs. The time required for a single analysis amounts to 14 min. The response was linear for the drug concentration ranges of 6.20–420.00, 0.80–80.00, 0.80–64.00, 2–40.00, 1.20–240.00, and 3.20–64.00, µg mL−1 for PA, CA, DO, ET, PP, and DH, respectively. Accuracy values for the method ranged from 99.40–101.94, 98.48–101.13, 98.97–100.54, 96.47–102.43, 98.92–101.82, and 97.63–100.45% for PA, CA, DO, ET, PP, and DH, respectively.