A Convenient Synthesis of Cyanine Dyes: Reagents for the Labeling of Biomolecules

Abstract

Abstract Four tetramethylindo(di)carbocyanine‐derived carboxylicacids have been prepared using a modified one‐pot procedure for the dye assembly. The acids were converted into oxysuccinimide esters and phosphoramidite reagents. The efficiency of the reagents in the labeling of oligonucleotides and proteins has been demonstrated. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)