Benzo[<i>a</i>]pyrene and zinc solubilization by digestive fluids of benthic invertebrates—a cross-phyletic study

Abstract

Contaminant bioavailability via digestive exposure was examined for 18 species of marine benthic invertebrates, using incubation of digestive fluids with sediments that were spiked with either radiolabeled benzo-[a]-pyrene (BaP) or zinc. Interphyletic trends in contaminant solubilization were compared with measures of digestive biochemistry, including enzyme activities, surfactancy, pH, and fluid phase organic carbon, amino acids, and lipids. Contaminant solubilization ranged from values equal to that of a seawater control to as much as an order of magnitude higher but were lower than those obtained with commonly used chemical extractants. Digestive fluids from echinoderms and a cnidarian tended to be relatively weak, those from polychaetes and echiurans were relatively strong, and those from taxa such as sipunculans and mollusks were intermediate. These trends correlated strongly with concentrations or activities of digestive biochemicals but not with pH. These correlations are consistent with previous work on mechanisms of digestive solubilization of polycyclic aromatic hydrocarbons (PAH) and metals, though strong covariance among digestive parameters does not allow this approach to be used for identification of specific mechanism(s).