Metabolism Studies of an Azo Dye and Pigment in the Hamster Based on Analysis of the Urine for Potentially Carcinogenic Aromatic Amine Metabolites

Abstract

administered to hamsters to determine whether these chemicals:are cleaved to potentially carcinogenic aromatic amines. These metabolism studies were Safety and Health and the Food and Drug Adminis-tration to assess the hazards that may occur if workers in the dye and pigment industries are occupationally exposed to these chemicals. Sensitive and specific assays of the urine by parallel electron-capture gas chromatography and high pressure liquid chromato-graphy revea!ed,the preseqce O! high levels, Of I~enzi, 'dine, monoacetylbenzidiqi~:~:~diacetYJb~qzidine,. ~4~': aminobiphenyl, and alkaliri~ " I?ydrq!yZable conjugates of benzidine and 4,aminobipheny! during the first eight hours of excretion after " administration of. Direc { BlaCk Sti l l presentiffthe urine at thet--e that the experiment: