Using Shape Information and Dark Paths for Automatic Recognition of Touching and Overlapping Chromosomes in G-Band Images

Abstract

Nowadays, Karyotype analysis is frequently used in cytogenetics. It is a time-consuming and repetitive work therefore an automatic analysis can greatly be valued. In this research, an automatic method is presented. Firstly, a proposed locally adaptive thresholding method is used to segment chromosome clusters. Then, the clusters is divided into two main categories including, single chromosomes and multichromosome clusters based on geometric shape of clusters. In the next step, each extracted cluster is investigated to find the dark paths in order to detect touching chromosomes.