Relationship between lactate dehydrogenase and myeloperoxidase levels in human gingival crevicular fluid and clinical and microbial measurements

TLDR

The study aimed to assess whether gingival crevicular fluid levels of lactate dehydrogenase and myeloperoxidase correlate with periodontal clinical and microbial parameters, and whether a single root‑planing session alters these enzyme levels and associated disease indicators. In a cross‑sectional cohort of 15 moderate‑to‑severe periodontitis patients and a longitudinal cohort of 12 patients, GCF from one healthy and two diseased sites per subject was sampled to quantify LDH and MPO before and after root planning. Elevated GCF LDH and MPO were strongly linked to worse clinical and microbial disease, root planning lowered both enzymes and disease signs, and LDH returned to baseline at three months while clinical signs persisted, suggesting a subclinical pathology marker.

Abstract

Abstract The present study was designed to determine, in a cross‐sectional study, whether there was any relationship between levels of lactate dehydrogenase (LDH) and myeloperoxidase (MPO) in gingival crevicular fluid (GCF) and clinical periodontal status or microbial parameters. Another objective was to determine, in a longitudinal study, the effect of a single session of root planning on GCF levels of LDH and MPO and the relation to changes in clinical and microbial measurements. 15 and 12 test subjects with moderate to severe periodontal disease were seen in the cross‐sectional and longitudinal study, respectively. 1 healthy and 2 diseased sites were evaluated in each subject. Higher LDH and MPO levels in GCF were closely associated with higher clinical and microbial signs of periodontal disease. Root planning was effective in reducing these enzymes in GCF, with an accompanying decrease in clinical and microbial signs associated with disease. The return of LDH to baseline levels at 3 months after instrumentation, without a corresponding return of clinical signs of disease, may serve as a marker for subclinical periodontal pathology.