Archives of Andrology · 2007 · 11 citations · 13 references
MedicineImmunologyPathologyAutoimmunityImmunopathologyInflammationAutoimmune Disease PathogenesisAutoimmune Disease PathologyAutoimmune InflammationImmune-mediated DiseaseMammalian ImmunologyProstate InflammationAbacterial ProstatitisAutoimmune ProstatitisDiphtheria-tetanus-pertussis VaccinationNitric OxideNitric Oxide Synthase RegulationProstatic InflammationRna In Situ HybridizationTetanus-diphtheria Vaccination
Male rats were immunized with prostate tissue homogenate supernate (PTHS) of male rats with complete Freund's adjuvant (CFA) intra dermal in the multiple points and simultaneously immunized with 0.5 ml Pertussis-Diphtheria-Tetanus (PDT) vaccine intra peritonea on 0 and 30th day. At the 45th day after first immunization, animals were sacrificed and a series of examinations such as HE stain, assay of TNF-alpha by ELISA and assay of inducible nitric oxide synthase (iNOS) mRNA by in-situ hybridization (ISH) were taken. We observed that there was a remarkable up-regulation of TNF-alpha expression in the high dosage model group. The results of macropathology, histopathology and iNOS ISH also revealed the same tendency. This experimental procedure is effective to induce chronic abacterial prostatitis (CAP).
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