Determination of Nitrate and Nitrite in Blood and Urine by Chemiluminescence

Abstract

Methodology has been developed for the rapid determination of nitrate and nitrite in blood and urine. In solution, these species are selectively reduced to nitric oxide, which is outgassed and determined via its gas phase chemiluminescence reaction with ozone. Differentiation between nitrate and nitrite is obtained by careful selection of reducing agents and conditions.