Datura Aqueous Leaf Extract Enhances Cytotoxicity via Metabolic Oxidative Stress on Different Human Cancer Cells

Abstract

This study was designed to evaluate the cytotoxic effect ﺔﺘﺒﻧ قاروﻷ ﻲﺋﺎﻤﻟا ﺺﻠﺨﺘﺴﻤﻟا ﺔﻴﻤﺳ ىﺪﻣ ﻢﻴﻘﺘﻟ ﺔﺳارﺪﻟا ﻩﺬه ﺖﻤﻤﺻ of aqueous Datura stramonium leaf extract on different human cancer cell lines in vitro. Breast (MDA-MB231), head, neck (FaDu), and lung (A549) cancer cell lines were treated with 1 mg/ mL of Datura aqueous extract for 24 and 48 hours. Exposure of MDA-MB231 and FaDu cells to the extract for 24 hours resulted in a significant decrease in cell survival. Same effect was seen with all cell lines exposed to the Datura aqueous extract for 48 hours. Treatment with Datura aqueous extract also caused perturbations in parameters indicative of oxidative stress, including increased glutathione disulfide (GSSG) in FaDu cells treated for 48 hours. Additionally, an increase on the redox sensitive enzymes was seen in MnSOD and HO-1 on A549 cells, treated with Datura aqueous extract for 24 and 48 hours. The results may suggest therapeutic potential of Datura aqueous leaf extract for the treatment of different types of cancer. Further investigations are needed to verify whether this cytotoxic effect occurs in vivo. مﻮﻴﻧﻮﻣاﺮﺘﺳا ةرﻮﺗاﺪﻟا ) Datura stramonium