Limitations and Practical Problems in Enzyme Linked Immunosorbent Assays .

Abstract

The major factors that limit the performance of enzyme-linked immunosorbent assays (ELISAs) have been described. Different variations have been explored for specific applications. Some of the common problems that are encountered while carrying out ELISAs have been highlighted. Suggestions have been made to overcome the difficulties and to improve the reliability of the enzyme immuno assays.