Antigen‐binding specificity of anti‐αGal reagents determined by solid‐phase glycolipid‐binding assays. A complete lack of αGal glycolipid reactivity in α1,3GalT‐KO pig small intestine

Abstract

Different anti-Gal reagents vary in their carbohydrate epitope specificity. Mono-/polyclonal Abs and lectins have different carbohydrate epitope fine specificity toward pig glycolipids as well as purified Galα1,3nLc4, and iGb3. Despite the difference in αGal specificity, all reagents were completely non-reactive with glycolipids isolated from α1,3GalT-KO pig small intestine.