Prevalence and antibiotic resistance of Staphylococcus strains isolated from meat products sold in Abidjan streets (Ivory Coast)

Abstract

Our study aimed to carry out the prevalence and antibiotic resistance of the coagulase positive and negative Staphylococcus isolated from meat product sold in streets in Abidjan (Ivory Coast). Two hundred and forty (240) samples from three kind of meat product (beef, pork and chickens) were collected in four popular communes (Abobo, Adjamé, Treichville and Yopougon) of Abidjan. These samples were composed of 80 samples of each kind of meat. After seeding on appropriate medium, suspectedStaphylococcus strains were preliminary identified using API Staph protocol. The exact bacteria identity was confirmed by MALDI-TOF mass spectrometry. The Staphylococcusstrains susceptibility to 18 antibiotics was determined using the disc diffusion method on Mueller Hinton medium. Out of the 240 tested samples, 96 Staphylococcus strains were isolated and identified. The coagulase positive specie isolated was Staphylococcus aureus with 19/96 (19.79%). Among the 77 coagulase negative strains, S. sciuri (32/77) was the most isolated followed by S. simulans (15/77). The highest resistance level was observed with erythromycin (100% for coagulase positive and 69.5% for coagulase negative). None resistance was observed with imipenem. The observed resistance to antibiotics of Staphylococcus strains suggests that the streets meat products sold at Abidjan are not appropriate and can be able to present a public health danger for the consumer.   Key words: Staphylococcus, coagulases, MALDI-TOF-MS, antibiotics, meat products, Ivory Coast.