In Vitro Selection of Structure‐Switching Signaling Aptamers

Abstract

Picking out probes: A novel approach permits isolation of standard DNA aptamers by in vitro selection and conversion into fluorescence signaling probes. This method comprises the isolation of DNA aptamers capable of duplex-to-complex structure switching and labeling the derived aptamers with a pair of short DNA strands with a fluorophore F and a quencher Q to create a reporter system for real-time sensing (see picture). Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2005/z461848_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.