Nonribosomal Peptide Biosynthesis: Point Mutations and Module Skipping Lead to Chemical Diversity

Abstract

Big effects from small changes: In a comparative analysis of the nonribosomal peptide synthetases (NRPSs) from the biosynthetic pathways of two highly related myxobacterial lipopeptides, module skipping and point mutations are directly correlated to structural variations in these natural products. The skipping process during myxochromide S biosynthesis was characterized biochemically and represents the first example of a module skipping in NRPS systems. Supporting information, including experimental data, for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2006/z503737_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.