Publication | Closed Access
THE INHIBITION OF TRYPTOPHAN PYRROLASE BY ALLOPURINOL, AN INHIBITOR OF XANTHINE OXIDASE
45
Citations
0
References
1967
Year
Molecular PharmacologyTryptophan PyrrolaseInhibitory ActivityAllergyBiochemistryPharmacological StudyGastrointestinal PharmacologyMedicineClinical PharmacologyPlasma Tryptophan LevelsPharmacotherapyTryptophan Pyrrolase ActivityExperimental PharmacologyPharmacologyRedox BiologyPharmacokineticsDrug DiscoveryOxidative Stress
The effect of allopurinol (4-hydroxypyrazolo(3,4-d)pyrimidine) on tryptophan pyrrolase activity of rats and mice was determined. After a single intraperitoneal injection of allopurinol at levels equal to or slightly greater than those given orally for the treatment of gout in humans, the drug was found to be an inhibitor of tryptophan pyrrolase activity. After injection of allopurinol, rats excreted significantly less kynurenine, but the drug did not alter plasma tryptophan levels. A study was made of the in vitro inhibition of tryptophan pyrrolase by allopurinol. The drug was found to be a very potent noncompetitive inhibitor of tryptophan pyrrolase with a K i value of 8 × 10 −7 M. Allopurinol at 1.5 × 10 −6 M concentration inhibited the enzyme 50%.