Production of Pectin Lyase by Colletotrichum lindemuthianum in Culture and in Infected Bean (Phaseolus vulgaris) Tissue

Abstract

Colletotrichum lindemuthianum race ? secreted two forms of pectin lyase, having pI values of 8·2 and 9·7, when grown in culture with sodium polypectate or isolated Phaseolus vulgaris hypocotyl cell walls as the main source of carbon. A single form of polygalacturonase was also present in both media. A pectin lyase, with a pI value of 9·7 was detected in necrotic P. vulgaris tissue infected with race ?, but polygalacturonase activity was absent.