Cellular Toxicity of Various Inhalable Metal Nanoparticles on Human Alveolar Epithelial Cells

TLDR

Nanoparticles can penetrate organisms via inhalation and may be more toxic due to their large surface area and unique properties, yet detailed studies on how size, structure, and composition influence toxicity are needed. The study aimed to evaluate in vitro biological effects of various inhalable metallic nanoparticles (TiO₂, Ag, Al, Zn, Ni). Human alveolar epithelial A549 cells were exposed to different concentrations of TiO₂, Ag, Al, Zn, and Ni nanoparticles for 24 h, and morphological damage was observed in a dose‑dependent manner, ranking m‑TiO₂ > n‑TiO₂ > m‑silica ≫ n‑Ni ≈ n‑Zn ≈ n‑Ag ≈ n‑Al. The nanoparticles induced dose‑dependent apoptotic damage, with n‑Zn causing the most apoptosis, followed by n‑Ni, m‑silica, and lower levels for n‑TiO₂, m‑TiO₂, n‑Al, and n‑Ag; DNA fragmentation mirrored this pattern, TiO₂ uptake was limited to TiO₂ particles, ROS was significant only with n‑Zn, and overall the results demonstrate that TiO₂, Ag, Al, Zn, and Ni can damage alveolar cells directly or indirectly.

Abstract

Nanoparticles (NPs) have a greater potential to travel through an organism via inhalation than any other larger particles, and could be more toxic due to their larger surface area and specific structural/chemical properties. The aim of this study was to evaluate in vitro biological effects of various inhalable metallic NPs (TiO2, Ag, Al, Zn, Ni). Human alveolar epithelial cells (A549) were exposed to various concentrations of NPs for 24 h. The extent of morphological damage was in the order of m-TiO2 > n-TiO2 > m-silica ≫ n-Ni ≈ n-Zn ≈ n-Ag ≈ n-Al and was affected in a dose-dependent manner. The extent of apoptotic damage measured with two-color flow cytometry was in the order of n-Zn > n- Ni > m-silica ≫ n- TiO2 > m- TiO2 > n-Al > n-Ag. The extent of apoptotic damage measured with DNA fragmentation was in the order of n-Zn ≈ m-silica > n- Ni ≫ m- TiO2 ≈ n- TiO2 ≈ n-Al > n-Ag, indicating no significant difference in the damages by both m-TiO2 and n-TiO2. The extents of apoptotic damages were also affected in a dose-dependent manner. Uptake of no other NPs but n-TiO2 and m-TiO2 into the cells was observed after 24 h exposure. The intracellular generation of ROS was significant with n-Zn but not with the other particles. These results demonstrated that various inhalable metallic NPs (TiO2, Ag, Al, Zn, Ni) could cause cell damages directly or indirectly. More detailed studies on the influence of size, structure, and composition of the NPs are needed to better understand their toxic mechanisms.