The Metabolism of 2-Oxogluconate by Pseudomonas aeruginosa

Abstract

SUMMARY: The 2-oxogluconate kinase and 2-oxogIuconate 6-phosphate reductase of Pseudomonas aeruginosa were purified approx. 100-fold. The activities of these enzymes, and a 2-oxogluconate transport system, were induced when the organism was grown on glucose, gluconate or 2-oxogluconate but were absent when the organism was grown on glycerol, succinate or citrate. Gluconate dehydrogenase is membrane-bound and acts extracellularly.