DETERMINATION OF HUMAN PLATELET SURVIVAL UTILIZING C14-LABELED SEROTONIN*

Abstract

All of the methods available for estimation of platelet survival have certain disadvantages. Iso- lation and considerable manipulation of platelets in vitro are necessary with the Cr51 technique (1, 2). The p32 (3), diisopropylfluorophosphate32 (DFP32) (4), or S35 (5) methods are all in vivo labeling procedures. Studies with p32 or S35 re- quire special subjects for study (patients with polycythemia vera or leukemia). Finally, both autologous and homologous platelet survival can- not be studied with facility.