Detection of Enzymatic Activity by PARACEST MRI: A General Approach to Target a Large Variety of Enzymes

Abstract

Perfect timing: A “smart” pro-PARACEST agent has been designed to detect β-galactosidase activity. Upon enzymatic attack, the self-immolative benzyloxycarbamate linker bearing the enzyme-specific substrate is cleaved and yields [Yb(dota-NH2)]−, which is endowed with a PARACEST effect. This pro-PARACEST agent is the first representative of a family of molecular imaging probes for the specific detection of a large variety of enzymatic activities using PARACEST. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2008/z800809_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.