A Ser315Thr Substitution in KatG Is Predominant in Genetically Heterogeneous Multidrug-Resistant <i>Mycobacterium tuberculosis</i> Isolates Originating from the St. Petersburg Area in Russia

Abstract

Parts of katG and rpoB from 27 Russian Mycobacterium tuberculosis isolates were sequenced to detect mutations causing resistance to isoniazid (INH) and rifampin (RMP), respectively. All 24 INH-resistant isolates had a mutated katG, and 22 of them (91.7%) carried a mutation coding for a Ser315Thr shift. An rpoB mutation was noted for each of the 21 RMP-resistant isolates, with Ser531Leu being the most prevalent change encoded. Only two isolates had identical IS6110 fingerprints.