Suicide vectors for antibiotic marker exchange and rapid generation of multiple knockout mutants by allelic exchange in Gram-negative bacteria

One-step inactivation of chromosomal genes in <i>Escherichia coli</i> K-12 using PCR products

Kirill A. Datsenko, Barry L. Wanner · Proceedings of the National Academy of Sciences · 2000 · 15.2K citations · Full text

Life Sciences, Genetics, Molecular Genetics +17

Studies on transformation of Escherichia coli with plasmids

Douglas Hanahan · Journal of Molecular Biology · 1983 · 11.3K citations

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A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria

Reinhard Simon, U. B. Priefer, Alfred Pühler · Bio/Technology · 1983 · 7.3K citations

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A rapid boiling method for the preparation of bacterial plasmids

David S. Holmes, Michael E. Quigley · Analytical Biochemistry · 1981 · 3.3K citations

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A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR

Virginia L. Miller, John J. Mekalanos · Journal of Bacteriology · 1988 · 2.1K citations · Full text

Life Sciences, Genetics, Molecular Biology +17