Genomics‐Driven Discovery of Burkholderic Acid, a Noncanonical, Cryptic Polyketide from Human Pathogenic <i>Burkholderia</i> Species

Abstract

Biosynthetic secrets unveiled: Targeted promoter exchange in a cryptic biosynthesis gene cluster conserved among certain pathogenic Burkholderia species yielded a highly unstable, structurally unprecedented polyketide, burkholderic acid (1). Labeling experiments, gene knock-outs, and bioinformatics analyses grant first insights into a fascinating polyketide pathway. BurA is an unusual nonribosomal peptide synthetase/polyketide synthase featuring internal thioesterase domains. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.