Ultrasensitive Electrochemical DNA Biosensors Based on the Detection of a Highly Characteristic Solid‐State Process

Abstract

Peptide nucleic acid probes are used in conjunction with amine-functionalized positively charged Ag nanoparticles to implement an ultrasensitive electrochemical DNA-sensing strategy (see image). Ag nanoparticles function as electroactive labels that can be detected through a characteristic solid-state Ag/AgCl reaction. Detection of a short H5N1 bird flu virus oligonucleotide demonstrates this strategy. A detection limit of 10 fM is achieved.