PATHOLOGICAL CHANGES IN THE ACUTE PHASE OF STREPTOZOTOCIN-INDUCED DIABETIC RATS

Abstract

This study aimed at investigating the acute phase of pathological changes in streptozotocin-induced diabetic rats with varying glucose levels. Eighteen rats, six control, six D1 group (with glucose level of 200-300 mg/dl), and six D2 group (with glucose level over 300 mg/dl), were used. They were euthanasied on the 14 th d after streptozotocin administration. Samples taken from internal organs were examined under a light microscope after routine histopathology processes. The changes in the organs were scored as light, mild and severe. The glomerulus, Bowman’s space, and renal corpuscle areas were calculated. There were significant differences between THE diabetic groups and control in terms of degenerative lesions in the pancreas, liver, spleen, and kidneys. It was found that increasing the area of Bowman’s space was directly proportional to blood glucose level. There was a significant difference between D1 and D2 in terms of the number of apoptotic cells in the spleen and there was an increase in apoptosis. It was concluded that the increased width of the Bowman’s space in the acute phase of type-I diabetes, and the changes observed in the other organs, would be useful for further studies on diabetes.