Journal of Clinical Investigation · 2009 · 140 citations · 27 references
Life SciencesMolecular BiologyOncologyGene ExpressionGene Expression AnalysisTranscriptomicsCancer BiomarkersDifferential Gene Expression AnalysisExpression Signature DiscoveryGene Expression ProfilingGene Fusions In CancerTranscriptomic ProfilingAcute Myeloid Leukemia BiologyAcute Promyelocytic LeukemiaGene Expression Signature MatchingLeukemia-associated Fusion GenesMicroarray AnalysisPml-rara Fusion GeneRna Expression Signature AnalysisSingle-cell Expression Signatures
Acute promyelocytic leukemia (APL) is characterized by the t(15;17) chromosomal translocation, which results in fusion of the retinoic acid receptor alpha (RARA) gene to another gene, most commonly promyelocytic leukemia (PML). The resulting fusion protein, PML-RARA, initiates APL, which is a subtype (M3) of acute myeloid leukemia (AML). In this report, we identify a gene expression signature that is specific to M3 samples; it was not found in other AML subtypes and did not simply represent the normal gene expression pattern of primary promyelocytes. To validate this signature for a large number of genes, we tested a recently developed high throughput digital technology (NanoString nCounter). Nearly all of the genes tested demonstrated highly significant concordance with our microarray data (P < 0.05). The validated gene signature reliably identified M3 samples in 2 other AML datasets, and the validated genes were substantially enriched in our mouse model of APL, but not in a cell line that inducibly expressed PML-RARA. These results demonstrate that nCounter is a highly reproducible, customizable system for mRNA quantification using limited amounts of clinical material, which provides a valuable tool for biomarker measurement in low-abundance patient samples.
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In silico prediction of protein-protein interactions in human macrophages
Oussema Souiai, Fatma Z. Guerfali, Slimane Ben Miled et al. · BMC Research Notes · 2014 · 11K citations · Full text